Temperature dependent xenobiotic metabolism and plasmatic responses in juveniles of solea senegalensis after ip injection to ibuprofen and carbamazepine



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TEMPERATURE DEPENDENT XENOBIOTIC METABOLISM AND PLASMATIC RESPONSES IN JUVENILES OF SOLEA SENEGALENSIS AFTER IP INJECTION TO IBUPROFEN AND CARBAMAZEPINE
A. González-Mira2 A. Martínez1, A. & A. Torreblanca2, M. Solé1,

1Institut de Ciencies del Mar (ICM-CSIC). Passeig marítim de la Barceloneta 37-49 08003 Barcelona, Spain. msole@icm.csic.es

2Departamento Biología Funcional y Antropología Física. Universitat de València. Dr. Moliner 50 Burjassot 46100 Valencia, Spain.

Abstract

Solea senegalensis juveniles were acclimatized at two temperatures, 15ºC and 20ºC, for a period up to 60 days. After that period, they were administered an intraperitoneal injection (IP) of the non-steroidal anti-inflammatory drug (NSAID) ibuprofen (IBU; 10 mg/kg; n=12) and the anti-convulsant drug carbamazepine (CBZ; 1 mg/kg; n=12). Two-days after the injection, plasma was withdrawn and fish organs dissected and stored at -80ºC for further analysis. Non-injected fish (control; n=16) and those injected only with the carrier (sunflower oil 1µl/g fish weight; n=12), also acclimated at each target temperature were used for comparison. Several hepatic and plasmatic biomarkers were measured at the two temperatures and conditions. Enzymatic activities at 15ºC were sensibly higher than at 20ºC mostly for CYP450 related activities (EROD, MROD, CECOD and BFCOD) and other microsomal parameters such as CbE and UDPGT whereas cytosolic antioxidant enzyme activities and GST did not show such a clear effect of temperature. Both drug treatments depressed cytosolic CbE (αNA) and microsomal UDPGT. CBZ injection elevated cytosolic GPX and microsomal CbE (pNPA) activity. Sunflower oil did not have an effect on the hepatic markers (except for CYP1A-related ones at 15ºC) but acted over some plasmatic parameters; thus, in the latter case contrasts were made against the carrier group. That is glucose, GOT and ɤ-GT were not affected by the treatment while lactate, ammonium and osmolality were affected by both pharmaceuticals, triglycerides were affected in a temperature dependent manner inclusive on the carrier group and creatinin was only affected by CBZ injection. Overall temperature had a more pronounced effect over hepatic parameters than the pharmaceuticals tested whereas plasmatic responses were more sensitive to treatment rather than to temperature acclimatation.


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